What happens when you treat cells with trichloroacetic acid at 37C, 1h?

29 September 2016 6,126 4 View

How Can I dissolve silica nanoparticles for Si detection in ICP-OES?

We want to study the biodistribution of our silica nanoparticles in mice. After injection of Si nanoparticles we have extracted different organs and tissues. Now, we want to quantify the...

01 July 2014 4,433 7 View

I tried to transfect NIH3T3 with pIRESneo-GFP-Protein using XtreamGene HP and X9. However, it didn't work. Has anybody experience transfecting 3T3?

I tried using 2ug of plasmid and different ratios of XtreamGene (HP or X9) 3:1, 6:1. The efficiency of transfection is too low. In addition, selected clones died 24 or 48 h post-selection.

07 February 2013 9,430 12 View

I tried to transfect NIH3T3 with pIRESneo-GFP-Protein using XtreamGene HP and X9. However, it didn't work. Has anybody experience transfecting 3T3?

I tried using 2ug of plasmid and different ratios of XtreamGene (HP or X9) 3:1, 6:1. The efficiency of transfection is too low. In addition, selected clones died 24 or 48 h post-selection.

01 February 2013 4,892 13 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View