Can you help me on measurement of atmospheric extintion coefficient gases?

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E-commerce businesses, COVID-19 and Entrepreneurial Strategies (innovation, proactiveness and risk-taking)?

Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey

05 August 2024 5,332 3 View

Someone can help me with error prone PCR?

How can you regulate the number of random inserted mutations in error prone PCR?

14 May 2024 673 2 View

How long do human brain tissue samples last after protein isolation?

Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...

02 May 2024 9,588 3 View

Does anyone have a photo of the eggs of Harpagifer bispinis?

It's not a question, but if anyone has a photo of the eggs of the fish called Harpagifer bispinis, please share it with me.

28 April 2024 5,052 2 View

What can be the impact of the periodic publication of antimicrobial susceptibility patterns in analyzing the results of the samples?

The question is based on the article, “Tenets of Specimen Management in Diagnostic Microbiology” by Rajeshwar and Pathak. It seeks to understand how microbiologists can analyze specimens submitted...

09 April 2024 4,849 3 View

How to get successful bacterial plasmid growth?

I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...

08 April 2024 2,035 4 View

Is this debris on my MEF cells or is it contamination?

I’m concerned about the dots on my MEF cells and I’m worried that it could be contamination.

31 January 2024 5,963 0 View

How can I have a better transfection rate of 50% for my HT-22 hippocampal neuronal cells?

I am currently working with HT-22 cells by transfecting them with bacterial plasmids to perform IHC. However, we have not had much success with reaching a 50% transfection rate. I usually do about...

17 January 2024 7,802 6 View

How can I fix my transfected cells for immunofluorescence protocol?

Hello. I plan to do a simple immunofluorescence procedure with HT-22 cells. I plan to transfect the cells and fix them. I do not believe I will have time to come in on the weekend. Is it possible...

11 December 2023 6,640 2 View

Determine the maximum velocities of the two outer balls if they were initially at rest and the middle ball was moving with speed v0?

Three balls with masses m1, m2, m3 can slide without friction along a straight horizontal line, with ball 2 located between balls 1 and 3 (Fig.). It is known that m1 >> m2, m3 >> m2....

29 November 2023 7,902 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Who of all of you will help TPEOM to re-originate the Earths atmosphere to it original 5 atmospheric elements/gases?

Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...

06 August 2024 8,716 0 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

What are the best innovative materials for harvesting water in arid and semi-arid regions?

Water scarcity in arid and semi-arid regions necessitates the development of innovative solutions for water harvesting. Researchers and engineers have been exploring various advanced materials...

28 July 2024 594 6 View

"How to handle large negative geopotential values at the surface in reanalysis datasets?

I have observed in some cases there are large negative values of surface geopotential in Era5 and MERRA2, in most cases in regions with complex orography. Why this happens? How can i handle this?

27 July 2024 4,628 2 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View

Unit for GHG emission rate?

In most of the literature I am reading at the moment, the abbreviation μg m−2 h−1 is used to express mean annual fluxes of greenhouse gases. I am confused by the "h" and supposed it stands for...

27 June 2024 9,633 1 View