Where can I find information about contractual practices that stimulate performance in urban transport by bus?

I would like to learn more about the contract design of urban transport by bus that stimulate competition and better performance. Among the elements that I would like to learn more about...

02 April 2019 7,562 1 View

What is the reason for the wide price range of Carbon Nanotubes? How to assess the quality of CNTs?

Suppliers of carbon nanotubes (SWCNT, DWCNT, MWCNT) provide very different quotes for their product which, at an industrial level, should be related with their quality: - How to assess the quality...

05 December 2018 5,514 3 View

What is the best cellular phenotype to do stress fiber formation studies by phalloidin staining? Are HEK293 cells a good model for that?

We are attempting to conduct studies of stress fiber formation response assessed by fluorescent phalloidin staining upon activation of heterologous expressed receptors in HEK293 cells. We would...

11 December 2017 3,048 0 View

What happens when you treat cells with trichloroacetic acid at 37C, 1h?

29 September 2016 6,126 4 View

Is it correct to affirm that pure Al has better fatigue behavior/fracture toughness than common commercial Al alloys like 2024, 6061 and 7075?

20 February 2015 3,662 5 View

Has anybody worked with upright microscopes for whole cell patch clamp experiments?

I have read that upright microscopes are normally used for slices recordings. But I need the set up for whole cell experiments. My problem comes when I try to approach the pipette to the cell. I...

10 November 2014 744 9 View

How Can I dissolve silica nanoparticles for Si detection in ICP-OES?

We want to study the biodistribution of our silica nanoparticles in mice. After injection of Si nanoparticles we have extracted different organs and tissues. Now, we want to quantify the...

01 July 2014 4,433 7 View

I tried to transfect NIH3T3 with pIRESneo-GFP-Protein using XtreamGene HP and X9. However, it didn't work. Has anybody experience transfecting 3T3?

I tried using 2ug of plasmid and different ratios of XtreamGene (HP or X9) 3:1, 6:1. The efficiency of transfection is too low. In addition, selected clones died 24 or 48 h post-selection.

07 February 2013 9,430 12 View

I tried to transfect NIH3T3 with pIRESneo-GFP-Protein using XtreamGene HP and X9. However, it didn't work. Has anybody experience transfecting 3T3?

I tried using 2ug of plasmid and different ratios of XtreamGene (HP or X9) 3:1, 6:1. The efficiency of transfection is too low. In addition, selected clones died 24 or 48 h post-selection.

01 February 2013 4,892 13 View

Long-lasting non-toxic dye injection in the brain

I am doing virus (AAV vectors) injection in deep brain structures (100~200 nl) of mice and I would like to simultaneously inject a dye or a colorant that is non-toxic (as much as possible...

13 January 2013 1,618 30 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View