Any advice on selecting human or animal studies to conduct a systematic review?

Human studies are more relevant to the project, however there are more mouse studies because it is mostly carried on neuron progenitor cells to identify the effect of a virus in the development of...

11 January 2021 5,628 3 View

Any papers discussing the effect of Zika virus on neuron development and its effects on neuronal functions?

I am looking into this research, any interesting papers relating to this would be amazing. Papers on pregnant women and infants would be great. Thank you :)

30 December 2020 1,962 3 View

I need updated CTE Sports related research?

I am looking for epidemiologyCTE for 2019 or 2020. also looking for treatment/management studies studies identifying tau pathology on postmortem studies. I have a few but struggling to find more...

28 October 2020 624 3 View

What PCR and real-time PCR machines perform best?

Hi everyone, I am equipping a new molecular biology/microbiology laboratory and I need to purchase a PCR and/or a real-time PCR machine. So I'm looking for suggestions. Is there a new model that...

11 October 2020 9,791 17 View

Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligo nucleotide in solid phase?

Hi, anybody help me? Why is anhydrous acetonitrile used to dissolve amidites in the synthesis of oligonucleotide in solid phase? What's the consequences?

28 July 2020 7,993 3 View

What the best way to improve Illumina genomic assembly with nanopore metagenomic data?

I have the Illumina reads and assembly of my bacteria from pure culture DNA sequencing and the nanopore sequencing data from the metagenome (DNA of co-culture). Does anyone know the best way to...

14 July 2020 6,516 3 View

Which phenomenological mathematical models describing the dynamic behaviour of microorganisms in the same ecosystem seem to be the most widely used ?

Hi, As part of a year-round trinomial project, I am looking for information about phenomenological mathematical models describing the dynamic behaviour of several microorganisms in the same...

01 April 2020 9,365 2 View

How do I describe a SDS PAGE when i am specifically looking for p53 protein?

Do I look at the most prominent bands on the figure? or do I look at bands at level 53kDa, even though they are not the most prominent in the image? Would i also mention that multiple bands...

23 March 2020 2,445 3 View

Flow cytometry: How to apply compensation to environmental samples when using an e.coli antibody?

We are using flow cytometry to run e.coli antibodies in environmental water samples. What is the best way to apply compensation? Thanks!

10 March 2020 8,379 4 View

Model protein that I can use as replacement for antibodies ?

Buying antibodies for lab tests is expensive, therefore I would like to know which protein model I could use instead. Thank you in advance.

23 January 2020 4,077 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View