Is inoue buffer stable on 4 degrees? Can it be used after storage at such conditions?

I am using the Inoue buffer for the preparation of competent cells. Can it be used after storage on 4 degrees? The references recommend the usage of freezed aliquots or fresh buffer every time.

02 March 2019 9,745 1 View

Recommended PAGE gel percentage for 39 KDa protein?

09 October 2016 6,955 8 View

Does EDTA antigen retrieval method gives high background stains and citrate buffer gives low background?

Does using EDTA antigen retrieval buffer in immunohistochemistry gives high background stains and citrate buffer gives low background stain?

01 February 2016 158 1 View

How can I purify extracted DNA from cell debris?

I extracted DNA using extraction column. It appeared that there was a lot of cell debris in the extract. Lot of impurities appeared in the nanodrop specking. How can I remove the cellular debris...

06 July 2015 2,139 8 View

Does anyone have the protocol for culturing endometrial cells from a biopsy?

I would like to culture endometrial cells to confluence into culture dishes. I would like to know if anyone has a good protocol with the mention of the need of coated dishes or other special...

04 May 2015 935 4 View

Is Kozak sequence necessary for protein expression?

I am expressing a protein tyrosine kinase in mammalian cells. Is it necessary to incorporate a Kozak sequence before the ATG initiation codon to assure that the expression happens? My sequence...

04 May 2015 6,743 17 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View