I want to seed normal endometrial cells
It differs for various cell lines. You need have pre-experiments by using different concentrations to find optimized seeding number. Usually, each dish needs 500000-2000000 cells.
I am using the Inoue buffer for the preparation of competent cells. Can it be used after storage on 4 degrees? The references recommend the usage of freezed aliquots or fresh buffer every time.
02 March 2019 9,892 1 View
What is the optimal PAGE gel percentage for visualization of a 39 KDa protein? Can Acryl/bis 37.5/1 be used instead of 40% pure acrylamide?
09 October 2016 7,076 8 View
Does using EDTA antigen retrieval buffer in immunohistochemistry gives high background stains and citrate buffer gives low background stain?
01 February 2016 257 1 View
I extracted DNA using extraction column. It appeared that there was a lot of cell debris in the extract. Lot of impurities appeared in the nanodrop specking. How can I remove the cellular debris...
06 July 2015 2,245 8 View
I would like to culture endometrial cells to confluence into culture dishes. I would like to know if anyone has a good protocol with the mention of the need of coated dishes or other special...
04 May 2015 1,029 4 View
I am expressing a protein tyrosine kinase in mammalian cells. Is it necessary to incorporate a Kozak sequence before the ATG initiation codon to assure that the expression happens? My sequence...
04 May 2015 7,085 17 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
