Does using EDTA antigen retrieval buffer in immunohistochemistry gives high background stains and citrate buffer gives low background stain?
Martyn Alun Sharpe
My recipe
Xylene
Graded Ethanol
PBS 0.1% Tween X-100
20-minute Steam Bath NaCitrate, pH 6.0
Wash PBS
1-hour DAKO Protein Block
1.8% H2O2 5 minutes;
1% Periodate 5 minutes;
0.02% NaBH4 2 minutes
PBS 0.1% Tween X-100
Primary IgG 1:100 1 hour
PBS 0.1% Tween X-100
HiDef HRP System
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