Emile Alghoul

7 Questions 13 Answers 0 Followers

Questions related from Emile Alghoul

Is inoue buffer stable on 4 degrees? Can it be used after storage at such conditions?

I am using the Inoue buffer for the preparation of competent cells. Can it be used after storage on 4 degrees? The references recommend the usage of freezed aliquots or fresh buffer every time.

03 March 2019 9,830 1 View

Recommended PAGE gel percentage for 39 KDa protein?

What is the optimal PAGE gel percentage for visualization of a 39 KDa protein? Can Acryl/bis 37.5/1 be used instead of 40% pure acrylamide?

10 October 2016 7,034 8 View

Does EDTA antigen retrieval method gives high background stains and citrate buffer gives low background?

Does using EDTA antigen retrieval buffer in immunohistochemistry gives high background stains and citrate buffer gives low background stain?

02 February 2016 201 1 View

How can I purify extracted DNA from cell debris?

I extracted DNA using extraction column. It appeared that there was a lot of cell debris in the extract. Lot of impurities appeared in the nanodrop specking. How can I remove the cellular debris...

07 July 2015 2,193 8 View

What is the best cell concentration to be seeded in a 10 cm gelatin coated dish?

I want to seed normal endometrial cells

05 May 2015 809 1 View

Does anyone have the protocol for culturing endometrial cells from a biopsy?

I would like to culture endometrial cells to confluence into culture dishes. I would like to know if anyone has a good protocol with the mention of the need of coated dishes or other special...

05 May 2015 983 4 View

Is Kozak sequence necessary for protein expression?

I am expressing a protein tyrosine kinase in mammalian cells. Is it necessary to incorporate a Kozak sequence before the ATG initiation codon to assure that the expression happens? My sequence...

05 May 2015 6,997 17 View