Hello!
I will use a myc-tagged version of a protein for co-immunoprecipitation studies. I have seen several commercial antibodies for this purpose, could you please recommend me which of these is the best in your experience?
Thank you!!!!
I've found that sigma's products work really well for both FLAG and MYC tagged proteins.
For example:
http://www.sigmaaldrich.com/catalog/product/sigma/ip0020?lang=en®ion=HK
Hi there,
Any 9E10 antibody derivative will do... Personally I use it from Santa-Cruz.
I used 9E10 as well!
Thank you!
You can try this kit:
http://www.piercenet.com/product/c-myc-tag-ip-co-ip-kits
Moreover, I would suggest to use PLA assay for the validation of your CoIP results.
Best wishes!
Sigma's
At ChromoTek you can get a free test sample and see for yourself:
http://www.chromotek.com/products/nano-traps/myc-trapr/myc-trapr-a/
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