I want to immobilize ammonium monooxygenase enzyme on conducting polymer.
What is a good procedure?
It depends on immobilization and storage conditions, from few days up to few years
Article Direct electron transfer from glucose oxidase immobilized on...
I want to detect apoptosis in rat brain after ischemia by using Annexin V FITC with Fluorescence Microscope. Can I do so? Please suggest protocol for brain tissue preparation for detection of...
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I want to estimate Glutamate from different region of the rat brain. Please suggest a detailed "step by step" procedure for the same by using HPLC with Electrochemical detector. I have C18 column...
10 November 2016 9,319 1 View
Dear Sir/Ma'am I have Blood flowmeter instrument from ADinstrument which works on the principle of Laser Doppler shifting. We have 2 types of electrodes - Surface and Needle. Can we measure...
07 August 2016 5,570 3 View
I want to make powder or some medicinal plant's leaves. Please suggest me detail procedure for the same
07 August 2015 4,529 0 View
I would like to induce global ischemia in a mice by using chemicals? Is it possible? How?
03 April 2015 7,969 4 View
Three important ions, sodium, chloride andpotassium are unequally distributed across the cellmembrane. Na+ and Cl– are more outside and K+ ismore inside. why not sodium ion concentration present...
07 August 2014 1,484 24 View
The SA node can be considered to be "self-firing". It repetitively goes through a depolarizing discharge and then repolarizes to fire again.Why does the SA node spontaneously fire at regular...
07 August 2014 3,779 7 View
My Problem:- I am working on ammonia sensor. I want to calibrate this sensor in a 1.2 L Airtight Chamber by Liquor Ammonia of 30% concentration and Specific Gravity 0.89. So, What amount (in...
31 December 2012 4,821 19 View
How we can use a manual UV spectrophotometer for quantitative analysis of any compound?
31 December 2012 5,344 5 View
Please help me to download the whole est sequence of Wuchereria bancrofti from ncbi.
31 December 2012 4,068 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟
09 July 2024 7,512 1 View
ماهو دور انزيم الكاتليز في النباتات؟
09 July 2024 5,824 2 View
what is the protocol for enzyme freeze drying?
09 July 2024 6,464 6 View
Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?
08 July 2024 2,285 2 View