I want to detect apoptosis in rat brain after ischemia by using Annexin V FITC with Fluorescence Microscope. Can I do so?
Please suggest protocol for brain tissue preparation for detection of apoptosis.
I want to estimate Glutamate from different region of the rat brain. Please suggest a detailed "step by step" procedure for the same by using HPLC with Electrochemical detector. I have C18 column...
10 November 2016 9,319 1 View
Dear Sir/Ma'am I have Blood flowmeter instrument from ADinstrument which works on the principle of Laser Doppler shifting. We have 2 types of electrodes - Surface and Needle. Can we measure...
07 August 2016 5,570 3 View
I want to make powder or some medicinal plant's leaves. Please suggest me detail procedure for the same
07 August 2015 4,529 0 View
I would like to induce global ischemia in a mice by using chemicals? Is it possible? How?
03 April 2015 7,969 4 View
Three important ions, sodium, chloride andpotassium are unequally distributed across the cellmembrane. Na+ and Cl– are more outside and K+ ismore inside. why not sodium ion concentration present...
07 August 2014 1,484 24 View
The SA node can be considered to be "self-firing". It repetitively goes through a depolarizing discharge and then repolarizes to fire again.Why does the SA node spontaneously fire at regular...
07 August 2014 3,779 7 View
My Problem:- I am working on ammonia sensor. I want to calibrate this sensor in a 1.2 L Airtight Chamber by Liquor Ammonia of 30% concentration and Specific Gravity 0.89. So, What amount (in...
31 December 2012 4,821 19 View
How we can use a manual UV spectrophotometer for quantitative analysis of any compound?
31 December 2012 5,344 5 View
I want to immobilize ammonium monooxygenase enzyme on conducting polymer. What is a good procedure?
31 December 2012 6,981 1 View
Please help me to download the whole est sequence of Wuchereria bancrofti from ncbi.
31 December 2012 4,068 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View