Gaurav Kumar

12 Questions 8 Answers 0 Followers

Questions related from Gaurav Kumar

Can we detect apoptosis by Annexin V FITC in rat brain?

I want to detect apoptosis in rat brain after ischemia by using Annexin V FITC with Fluorescence Microscope. Can I do so? Please suggest protocol for brain tissue preparation for detection of...

09 September 2017 5,730 0 View

How can I estimate the Glutamate using HPLC with ECD?

I want to estimate Glutamate from different region of the rat brain. Please suggest a detailed "step by step" procedure for the same by using HPLC with Electrochemical detector. I have C18 column...

11 November 2016 9,337 1 View

Can we measure Cerebral Blood Flow by placing the electrode on the Rat Cranium?

Dear Sir/Ma'am I have Blood flowmeter instrument from ADinstrument which works on the principle of Laser Doppler shifting. We have 2 types of electrodes - Surface and Needle. Can we measure...

08 August 2016 5,593 3 View

How we can obtain leaf powder from green leaves?

I want to make powder or some medicinal plant's leaves. Please suggest me detail procedure for the same

08 August 2015 4,542 0 View

Can we induce global ischemia in mice by using some chemicals?

I would like to induce global ischemia in a mice by using chemicals? Is it possible? How?

04 April 2015 7,984 4 View

Why does the SA node spontaneously fire at regular intervals?

The SA node can be considered to be "self-firing". It repetitively goes through a depolarizing discharge and then repolarizes to fire again.Why does the SA node spontaneously fire at regular...

08 August 2014 3,810 7 View

Why not sodium ion concentration present inside the cell instead of potassium ion?

Three important ions, sodium, chloride andpotassium are unequally distributed across the cellmembrane. Na+ and Cl– are more outside and K+ ismore inside. why not sodium ion concentration present...

08 August 2014 1,502 24 View

How we can download an est sequence?

Please help me to download the whole est sequence of Wuchereria bancrofti from ncbi.

01 January 2013 4,081 2 View

What is the approximate shelf life of a enzyme immobilized on a transducer?

I want to immobilize ammonium monooxygenase enzyme on conducting polymer. What is a good procedure?

01 January 2013 6,994 1 View

Is this the correct calculation for ppm calculation?

My Problem:- I am working on ammonia sensor. I want to calibrate this sensor in a 1.2 L Airtight Chamber by Liquor Ammonia of 30% concentration and Specific Gravity 0.89. So, What amount (in...

01 January 2013 4,851 19 View

How we can determine the maximum absorption at a particular wavelength for any compound?

How we can use a manual UV spectrophotometer for quantitative analysis of any compound?

01 January 2013 5,361 5 View

How we can calculate the concentration in ppm?

We have liquid Ammonia with a 30% (w/w) concentration. How much liquid ammonia is needed to make 1 ppm after evaporation in a 1.2 L chamber? Any suggestions?

12 December 2012 1,670 84 View