What is the alternative of Pen-Strep in cell culture?

More Shahid Mahmood's questions See All

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

What are the frameworks or methodologies to examine written academic ELF?

What should be the frameworks or methodologies to examine written academic ELF? I want to explore the linguistic features of written ELF in research articles.

23 July 2024 4,800 1 View

How can I define Planetary motion in ABAQUS CAE, how define motion of three rollers around a central axis and passive rotation about their own axis?

16 July 2024 4,145 1 View

Is the research on counselling of patients is cross sectional or clinical trail in psychiatry?

Since in Psychiatry , treatment is done through counselling and medicines are prescribed according to it, my question is what would be the most appropriate study design in the subject of psychiatry.

14 July 2024 8,240 2 View

How can I synthesize beads of silica?

Hi to all of you! My goal is to create synthetic silica beads. These beads ought to have a specified size. Could someone please advise on how to proceed or give me the articles I need to finish my...

02 July 2024 8,170 1 View

How to find the incident spectra in Lumerical FDTD?

Hello, I am having a difficult time finding the incident plane wave wavelength dependent spectra of the lights source to find the EQE. Please advise.

26 June 2024 7,274 1 View

Which other method other than Gas Chromatography can be used in the content determination of Alcohol in Pharmaceutical syrup?

Other than GC any other method might help.

26 June 2024 1,076 3 View

How to use dynamic phasor model in Digsilent?

I saw RMS and EMT simulation respectively in Digsilent, but my research focus is on dynamic phasor, so how i can do it? My model is a DFIG connect to IEEE39 system

15 May 2024 6,406 0 View

What is the procedure of Molecular Diagnosis of Friedreich Ataxia?

Hello I am seeking detailed protocols for the molecular diagnosis of Friedreich Ataxia. Best wishes Saqib

13 May 2024 1,448 2 View

How we can efficiently deposit cathode materials like NCA, NMC or LFP using Thermal Evaporation?

We are facing hurdles in achieving desired deposition rates while depositing cathode-based materials via thermal evaporation. Despite starting with a vacuum setup reaching up to 10^-7 torrs,...

03 May 2024 8,060 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View