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Hi, experts. I'm trying to expressed recombinant transmembrane protein incorporated with nucleophilic non-canonical amino acids(ncAAs) by genetic code expansion (GCE) in E.coli. I replace tyrosine...
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Hi, i am working on unnatural Amino Acid (UAAs) incorporated Insulin receptor substrate (IRS-1) expression and purification in BL21(DE3) strain. While i expressed my protein with UAA Glutathion...
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Hey Researchers, i am working with transformation of membrane protein by using BL21(DE) competent cell. i transformed plasmid (pET28a(FLAG, His tag) and co-transformed with pULTRA-ambryx. i...
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Hello, I am working on scFV phage display library. I got positive monoclonal from Phage display library. Now i did Phage-antibody competitive ELISA. i got following surprising results. 1) Phage...
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I'm doing scFv phage display library panning with photocross linked protien. after panning i performed colony PCR for confirmation of scFv length. i got various short length fragment on gel, only...
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I did Western Blot (WB) with Photo-cross linked protein. I had some unusual thing (maybe for me). When i added ECL substrate on membrane. The signals were getting weak with in just 1 mint and...
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Which techniques are more accurate and far-better for miRNA expression analysis. Is micro array or miRNA sequences tech? if RNA Sequence tech then please share article for initial understanding or...
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Anything or alternative antibiotic that i use instead of Pen-Strep. Any other antibiotic that use for cell culture.
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