What is poly T adapter in poly (A) tailing assay for miRNA detection. Is poly T adapter and university RT primer same or not?
I have a netCDF4 (.nc) file having ocean SST data, with coordinates (lat, lon, time). I want to predict and plot maps for the future. How can I do this using python? Please recommend a python...
27 October 2023 8,614 3 View
I have a monthly netCDF4 file containing chlorophyll-a values, and I aim to forecast these values using time series analysis. My approach involves computing monthly spatial averages for this...
21 October 2023 8,299 1 View
How to design the primers for poly (A) tailing assay
06 July 2023 5,172 1 View
What is the best way to synthesize cDNA; using poly A kit or stem loop PCR? Why is that
13 June 2023 4,626 4 View
Protocol for testing COD in pharmaceutical industry waste water
04 February 2023 3,393 7 View
Are there any reference system for energy level distribution in metal oxides in these ions? I know there id Dieke diagram for fluorides and chlorides. any similar system/chart or diagram for metal...
25 July 2021 3,142 2 View
I need to dissolve a few fatty acids of C10 to C20 such as myristic, stearic and arachidic acid for a PRO-DNA binding reaction to carry out an EMSA. I tried with DMSO, where I dissolved the acids...
29 November 2020 7,664 3 View
I am looking for a buffer composition other than MES buffer to carry out an EMSA. I need to maintain a low pH for a ligand binding assay, since the ligand believed to be effective in the pH range...
14 July 2019 3,330 3 View
I have data for SNP with genotypes TT, TC and CC. I want to check the effect of these individual genotypes on the disease outcome, which is iron deficiency. How can I apply dominant, recessive...
03 March 2019 8,393 3 View
Accepted methodology, solvent for analysis, solvent ratios with time plan, black tea sample extraction procedure for minimum matrices and colour figments effects.
27 November 2015 4,901 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View