What is the best way to synthesize cDNA; using poly A kit or stem loop PCR? Why is that
The choice between using a poly A kit or stem loop PCR for cDNA synthesis depends on the specific requirements of your experiment.
The choice between the two methods depends on the type of RNA you are interested in studying. If you want to analyze mRNA and coding sequences, a poly A kit would be more appropriate. If you specifically want to analyze small non-coding RNA molecules like miRNAs, stem loop PCR is the preferred method.
Ultimately, the best way to synthesize cDNA depends on the specific goals and experimental requirements of your study.
thank you Hevar Neaz for the explanation. Could you please tell me, why stem-loop PCR is preferred for small RNA such as tDRs analysis
Dear Halinne Lokuge Thilakshi Chamanika Abeywickrama
Stem-loop PCR is preferred for tDRs analysis due to its specificity, efficient reverse transcription, protection against degradation, accurate quantification, low input requirements, and compatibility with downstream applications.
Dear Hevar Neaz thank you very much
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