Halinne Lokuge Thilakshi Chamanika Abeywickrama

7 Questions 5 Answers 0 Followers

Questions related from Halinne Lokuge Thilakshi Chamanika Abeywickrama

Immuno nothern protocol?

Can someone shared a protocol for immuno nothern blot (I am detecting biotin labelled tRNA derived fragment and detecting with an antibody, not with the probe). I tried according to Mishima E, Abe...

12 May 2025 2,933 2 View

What is poly T adapter in poly (A) tailing assay for miRNA detection. Are poly T adapter and university RT primer same or not?

What is poly T adapter in poly (A) tailing assay for miRNA detection. Is poly T adapter and university RT primer same or not?

07 July 2023 5,394 0 View

How to design the primers for poly (A) tailing assay ?

How to design the primers for poly (A) tailing assay

07 July 2023 5,211 1 View

What is the best way to synthesize cDNA; using poly A kit or stem loop PCR? Why is that?

What is the best way to synthesize cDNA; using poly A kit or stem loop PCR? Why is that

14 June 2023 4,670 4 View

Please recommend a validated procedure for testing chemical oxygen demand in industrial waste water?

Protocol for testing COD in pharmaceutical industry waste water

05 February 2023 3,433 7 View

Optimizing BgL2 digestion of Pjet DNA

I need to obtain high-quality pJETAsptRNA DNA plasmid to synthesize the oligos by using a T7 polymerase RNA synthesis kit. I am using Bgl2 digestion to access the plasmid DNA quality. But my DNA...

01 January 1970 5,645 1 View

Full length tRNA transcript synthesis by T7 polymerase

When I used 95bp PCR product (full-length tRNA with promoter), the T7 polymerase RNA synthesis kit (NEB) gave a lot of large bands (more than 200bp). Can you explain the reason and the way to...

01 January 1970 8,623 2 View