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Questions related from Halinne Lokuge Thilakshi Chamanika Abeywickrama
Can someone shared a protocol for immuno nothern blot (I am detecting biotin labelled tRNA derived fragment and detecting with an antibody, not with the probe). I tried according to Mishima E, Abe...
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What is poly T adapter in poly (A) tailing assay for miRNA detection. Is poly T adapter and university RT primer same or not?
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How to design the primers for poly (A) tailing assay
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Protocol for testing COD in pharmaceutical industry waste water
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I need to obtain high-quality pJETAsptRNA DNA plasmid to synthesize the oligos by using a T7 polymerase RNA synthesis kit. I am using Bgl2 digestion to access the plasmid DNA quality. But my DNA...
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When I used 95bp PCR product (full-length tRNA with promoter), the T7 polymerase RNA synthesis kit (NEB) gave a lot of large bands (more than 200bp). Can you explain the reason and the way to...
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