Any low pH buffers compatible with EMSA?

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Adam B Shapiro

Citrate or acetate.

https://www.aatbio.com/resources/buffer-preparations-and-recipes/

Antonina Kokisheva

Phosphate-citric buffer, pH=5,5

disodium phosphate (Na2HPO4 ) 1,61 g

citric acid ( HOOC-CH₂-CCOOH-CH₂-COOH ) 0,91 g

H20 to V=100 ml

Thanusha Abeywickrama

Thank you very much Adam and Antonina.