Any low pH buffers compatible with EMSA?

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Can I please know of methods to dissolve fatty acids for an EMSA?

I need to dissolve a few fatty acids of C10 to C20 such as myristic, stearic and arachidic acid for a PRO-DNA binding reaction to carry out an EMSA. I tried with DMSO, where I dissolved the acids...

29 November 2020 7,513 3 View

How can I analyse the data from a single nucleotide polymorphism under three genotypic test models in SPSS ?

I have data for SNP with genotypes TT, TC and CC. I want to check the effect of these individual genotypes on the disease outcome, which is iron deficiency. How can I apply dominant, recessive...

03 March 2019 8,300 3 View

Is there are any nanoparticles are directly used for wound healing purposes?

I want to study about nanoparticles targeting for wound healing purposes Which type of nanoparticles are specific?

03 September 2014 9,705 16 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Adam B Shapiro

Citrate or acetate.

https://www.aatbio.com/resources/buffer-preparations-and-recipes/

Antonina Kokisheva

Phosphate-citric buffer, pH=5,5

disodium phosphate (Na2HPO4 ) 1,61 g

citric acid ( HOOC-CH₂-CCOOH-CH₂-COOH ) 0,91 g

H20 to V=100 ml

Thanusha Abeywickrama

Thank you very much Adam and Antonina.