Accepted methodology, solvent for analysis, solvent ratios with time plan, black tea sample extraction procedure for minimum matrices and colour figments effects.
Are you trying this fungicide residue or deposit,no problem,you can mix the black tea ,in ice temp.. then take 5 gr for analysis best solvent is acetinitryl , 10 to 15 ml and for color sepration PSA in needed , in extraction procedure PH is important.
the solvent was acetonitryl , after extraction procedure you can inject in a relibele chromatographic system such as GC or GC/MS system for this fungicide GC/MS is suitable.