What is the size of the peptides absorbed through digestion of humans?

Physiology

05 June 2018 8,861 0 View

How many chances are available for the expression of polypeptide chains from a gene?

It is the basic question to test the knowledge of molecular biology.

01 February 2014 2,011 1 View

How to isolate osteopontin protein from cancer cell line?

There is no standard procedure for isolation of osteopontin from cell line culture.

09 October 2013 8,011 1 View

Which protein is responsible for the calcification of cancer cells?

Most probably osteopontin.

08 September 2013 2,618 3 View

How many net number of ATP molecules are produced from Palmitic acid during beta oxidation process?

The answer is 129. Explain

08 September 2013 7,530 11 View

Why lactate level decreased in plasma of cancer patients?

In breast cancer patients tissue homogenate showed increased level of lactate in breast cancer patients whereas plasma levels showed a decrease in lactate level compared to normal persons.

08 September 2013 6,705 1 View

When is a vaccine considered to be a good or a complete vaccine?

When a vaccine has an ability to stimulate Th cells, then it will be considered a good vaccine.

08 September 2013 2,909 6 View

What is the online tool used for structure refinement?

I had used SUMMA server for structure refinement.

07 August 2013 3,080 5 View

Why is thymine present in DNA instead of Uracil?

Due to aid the repair system to identify the mutated cytosine through the deamination process.

07 August 2013 8,639 9 View

Which online docking tool can be used for protein-protein docking?

In my experience, Cluspro, Firedock, Zdock, GRAMMX, VaskerLab and FroDock etc., were used for protein-protein docking.

07 August 2013 1,896 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View