It might be oligomeric or multimeric enzyme, and the shape might appear due to co-operative behaviour of different subunits.
Physiology
05 June 2018 9,004 0 View
It is the basic question to test the knowledge of molecular biology.
01 February 2014 2,140 1 View
There is no standard procedure for isolation of osteopontin from cell line culture.
09 October 2013 8,187 1 View
Most probably osteopontin.
08 September 2013 2,753 3 View
The answer is 129. Explain
08 September 2013 8,204 11 View
In breast cancer patients tissue homogenate showed increased level of lactate in breast cancer patients whereas plasma levels showed a decrease in lactate level compared to normal persons.
08 September 2013 6,834 1 View
When a vaccine has an ability to stimulate Th cells, then it will be considered a good vaccine.
08 September 2013 3,032 6 View
I had used SUMMA server for structure refinement.
07 August 2013 3,233 5 View
In my experience, Cluspro, Firedock, Zdock, GRAMMX, VaskerLab and FroDock etc., were used for protein-protein docking.
07 August 2013 2,065 2 View
Due to aid the repair system to identify the mutated cytosine through the deamination process.
07 August 2013 9,049 9 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View