How many chances are available for the expression of polypeptide chains from a gene?

It is the basic question to test the knowledge of molecular biology.

01 February 2014 2,011 1 View

How to isolate osteopontin protein from cancer cell line?

There is no standard procedure for isolation of osteopontin from cell line culture.

09 October 2013 8,011 1 View

Which protein is responsible for the calcification of cancer cells?

Most probably osteopontin.

08 September 2013 2,618 3 View

How many net number of ATP molecules are produced from Palmitic acid during beta oxidation process?

The answer is 129. Explain

08 September 2013 7,530 11 View

Why lactate level decreased in plasma of cancer patients?

In breast cancer patients tissue homogenate showed increased level of lactate in breast cancer patients whereas plasma levels showed a decrease in lactate level compared to normal persons.

08 September 2013 6,705 1 View

When is a vaccine considered to be a good or a complete vaccine?

When a vaccine has an ability to stimulate Th cells, then it will be considered a good vaccine.

08 September 2013 2,909 6 View

What is the online tool used for structure refinement?

I had used SUMMA server for structure refinement.

07 August 2013 3,080 5 View

What is nature of enzyme, if effect of substrate concentration on enzyme activity curve shows sigmoidal shape?

It might be oligomeric or multimeric enzyme, and the shape might appear due to co-operative behaviour of different subunits.

07 August 2013 5,166 0 View

Why is thymine present in DNA instead of Uracil?

Due to aid the repair system to identify the mutated cytosine through the deamination process.

07 August 2013 8,639 9 View

Which online docking tool can be used for protein-protein docking?

In my experience, Cluspro, Firedock, Zdock, GRAMMX, VaskerLab and FroDock etc., were used for protein-protein docking.

07 August 2013 1,896 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View