There is no standard procedure for isolation of osteopontin from cell line culture.
You should contact Prof. Vincent Castronono (Université de Liège; Belgium) who is registered on RG. He knows very well this area.
Best regards
Robert
Physiology
05 June 2018 8,861 0 View
It is the basic question to test the knowledge of molecular biology.
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Most probably osteopontin.
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The answer is 129. Explain
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In breast cancer patients tissue homogenate showed increased level of lactate in breast cancer patients whereas plasma levels showed a decrease in lactate level compared to normal persons.
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When a vaccine has an ability to stimulate Th cells, then it will be considered a good vaccine.
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I had used SUMMA server for structure refinement.
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It might be oligomeric or multimeric enzyme, and the shape might appear due to co-operative behaviour of different subunits.
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Due to aid the repair system to identify the mutated cytosine through the deamination process.
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In my experience, Cluspro, Firedock, Zdock, GRAMMX, VaskerLab and FroDock etc., were used for protein-protein docking.
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