Can anyone recommend me your cloning protocol that successfully works in your laboratory?

Dear fellow scientists!!! I have been doing cloning lately and have encountered some problems during my experiments, for example, I can get none or very few colonies after transformation into...

02 December 2020 2,271 11 View

Can Journal still publish my article after I send them mail to withdraw it?

Just Yesterday, I submitted an article in the International Journal of Advanced Science and Technology(ISSN: I mistakenly sent some other file instead of the original file. The file I sent wasn't...

18 July 2020 2,480 7 View

What is the best stock concentration and final concentration of Cobalt 2 chloride hexahydrate?

Hello Everyone, I have some questions related to a drug called Cobalt 2 chloride hexahydrate. I am currently preparing an experiment related to Hypoxia. I bought a drug called " Cobalt 2...

10 May 2020 4,841 4 View

Why do my cells cluster and form circular rings at the middle of the plate?

Hello Everyone, I have some questions related to cell plating in 6-well plate. I have plated my cells (miaPaca-2 and PANc-1 cell lines) in 6-well plates. The Media I use is DMEM (with 10% FBS...

08 March 2020 8,329 3 View

Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?

Hello, Everyone. I have some questions related to western blot as I am new to western blot. I tried to detect the LC3B, however I got diffused bands as I have attached in the file (Please find the...

12 November 2019 8,250 13 View

ESI-MS of Melanin at 375?

I have a naturally extract melanin sample and mass spectra gives M value of 375 (peaks for M+1, M+23, 2M+23). Could it be any possible variant of melanin? There is an additional peak at 223 which...

10 October 2019 3,630 3 View

Why the expression pattern in Real-time PCR keeps changing for the same primer?

Good morning, Everyone! I have some questions related to Real-time PCR. I have checked the expression of a microRNA (microRNA primer) in monolayer and spheroid cells. The first time I run the...

13 June 2019 4,395 12 View

Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?

I am looking for a standard protocol of how to design a good primer for RT-PCR. Could anyone recommend me the standard protocol of the design of (microRNA or gene) primers for RT-PCR as a reference?

13 June 2019 9,401 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View