Hello Everyone,
I have some questions related to a drug called Cobalt 2 chloride hexahydrate.
I am currently preparing an experiment related to Hypoxia. I bought a drug called " Cobalt 2 chloride hexahydrate" (from Sigma Aldrich) with the molecular weight of 237.9 g/mol and the total weight is 5g.
I want to know what is the best stock concentration should we make for this drug? Plus, how much H20 should we add to the drug ? and the optimal condition to store this stock concentration?
Furthermore, can you recommend me the best final concentration of this drug to mimic the Hypoxia environment? any ideas?
Dear Sv Mg, for such experiments, the cobalt(II)chloride hexahydrate, CoCl2x6H2O, is used in very low concentrations such as 150 µM as listed in Table 2 in the Review article shown below. The paper is available on ResearchGate. The molar mass of CoCl2x6H2O is 237.93 g/mole.
Article The use of cobalt chloride as a chemical hypoxia model: Coba...
Dear Sv Mg, 150 µM cobalt(II)chloride hexahydrate means that you dissolve 0.356 mg of CoCl2x6H2O in 1 liter of water.
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