CUDA Out Of Memory (OOM) error while using GPU?

I'm training a neural network (E3D-LSTM) for spatio temporal video prediction. It's quite a big network that has millions of parameters. The model is training perfectly when using my 12 CPU cores,...

25 December 2020 1,229 3 View

Is there any integrated genome browser available to see chloroplast gene expressions?

Dear colleagues, Good Day! I am looking for any public database for visualizing RNA-seq data of chloroplast genes. For ex. I want to see the expression of Polycistronic mRNA abundancies of genes...

20 December 2020 7,474 3 View

Can anyone recommend me your cloning protocol that successfully works in your laboratory?

Dear fellow scientists!!! I have been doing cloning lately and have encountered some problems during my experiments, for example, I can get none or very few colonies after transformation into...

02 December 2020 2,271 11 View

Need airfoil coordinates?

Hi all, I hope you all are doing well. I am working on the simulation of airfoils with boundary layer suction. I found a reference to validate my CFD results, as shown below. "Stanewsky E,...

06 September 2020 9,013 3 View

Can Journal still publish my article after I send them mail to withdraw it?

Just Yesterday, I submitted an article in the International Journal of Advanced Science and Technology(ISSN: I mistakenly sent some other file instead of the original file. The file I sent wasn't...

18 July 2020 2,480 7 View

Porous media coefficients and working fluid?

Hi all, I hope you all are doing well! I am wondering if the porous media coefficients will change if the working fluid changes. I have a tubular, U-shaped heat exchanger geometry where the...

01 July 2020 246 3 View

What is the best stock concentration and final concentration of Cobalt 2 chloride hexahydrate?

Hello Everyone, I have some questions related to a drug called Cobalt 2 chloride hexahydrate. I am currently preparing an experiment related to Hypoxia. I bought a drug called " Cobalt 2...

10 May 2020 4,841 4 View

What is the adiabatic heat transfer coefficient, and how to estimate it using CFD?

Hi everyone, I hope you all are doing well. Can anybody in the forum explain what adiabatic wall temperature and heat transfer coefficients are? More importantly, how to estimate it using CFD...

25 March 2020 4,644 11 View

Why do my cells cluster and form circular rings at the middle of the plate?

Hello Everyone, I have some questions related to cell plating in 6-well plate. I have plated my cells (miaPaca-2 and PANc-1 cell lines) in 6-well plates. The Media I use is DMEM (with 10% FBS...

08 March 2020 8,329 3 View

Can anyone explain why do we get diffused bands in western blot? and any trouble shooting techniques?

Hello, Everyone. I have some questions related to western blot as I am new to western blot. I tried to detect the LC3B, however I got diffused bands as I have attached in the file (Please find the...

12 November 2019 8,250 13 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

If glyphosate is used after sugarcane harvesting, what will be the effect on the germination of ratoon crop?

If glyphosate is used after sugarcane harvesting, what will be the effect on the germination of ratoon crop?

26 February 2020 8,638 9 View

Is there any relevant literature detailing extraction methods of simple sugars from plant material for LC-MS analysis?

For my research, I would like to extract glucose, sucrose, and fructose from the crowns of strawberry plants and quantify these sugars using LC-MS. However, I have not had much luck finding a...

11 February 2020 398 3 View

What is the best way to express Kruskal-Wallis test result visually (through graph)?

I have conducted an experiment regarding anthracnose disease of strawberry and the data collected for disease index appears to be non-normal distribution. As such, I conducted Kruskal-Wallis test...

11 December 2019 5,184 1 View

What internal control for qPCR of fungi detection in plants is recommended ?

I need to perform qPCR detection of specific fungal species in plant (strawberry). I need to include internal control (housekeeping gene) for plant, I have found that some people use COX gene, but...

11 July 2019 7,756 2 View

Biology and geometry: pentagon shape in strawberry?

I have just observed on the number of strawberries I was able to eat today that: -when you carefully take the leaves off the strawberry -the back of the strawberry shows a pentagonal shape See...

05 June 2019 395 5 View

Articles or Books about Strawberry plant architecture?

03 April 2019 8,724 2 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 7,547 3 View

How to extract total protein from strawberry fruit?

I have extracted protein from strawberry by 4% SDS+65mM DTT buffer and found good band in SDS PAGE but when I performed western blot, i didn't observed protein band. What is the reasons? I have...

03 April 2019 4,817 3 View

Please what is the peak retention time for glyphosate standards and HPLC method of identifying and quantifying glyphosate from animal tissues ?

i need urgent answer please

02 March 2019 8,692 2 View