i fermented hydrolyzed ulva by Bacillus subtilis for 48 h but it wasn't sufficient enough how can i increase the amount of fınal PHB
Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...
23 February 2021 1,908 2 View
I am looking for PCR primers for bacillus Sp.
15 February 2021 2,552 5 View
I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...
17 January 2021 5,099 4 View
I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...
09 January 2021 8,309 4 View
I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...
05 January 2021 3,860 2 View
Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...
20 December 2020 7,166 2 View
Hi. I need help to measure trypsin inhibitor activity in fermented soy and fermented oat samples. The problem is my samples were mixed with water with ratio 1:3 and 1:2 respectively, for...
13 December 2020 6,882 3 View
We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...
04 December 2020 7,657 6 View
Hi everyone! I would like to knockout a gene in the genome of Bacillus. To do that I tried to make a construct that has 1) a backbone, 2) a kanamycin resistance cassette, flanked by 3) the...
24 November 2020 489 1 View
The strain is Candida tropicalis. It has been stored in 4 degree refrigerator for a year. Now we want to see if it is possible to revive the strain and make it function again. I tried to culture...
10 November 2020 5,650 9 View