Can we use just only universal primer for invertebrates or do we need to develop or design primer for certain classes or group for the amplification method to be successful?
You can use ITS.2 primers :
Article Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA
Aissa Saidi I forgot to tell that we actually want to use CO1 gene as DNA marker for identification.
Here are some references for CO barcodes in nematodes:
Article Exploring the Use of Cytochrome Oxidase c Subunit 1 (COI) fo...
Article Disentangling taxonomy within the Rhabditis (Pellioditis) ma...
Article Barcoding marine nematodes: An improved set of nematode 18S ...
Article Exploitation of archived marine nematodes - A hot lysis DNA ...
Article An integrated approach to fast and informative morphological...
The "universal" COI primers work for many inverts, but not for all. You should scan the literature for the right primers. There are plenty of papers on flukes and nematodes. Primers are cheap to purchase. If you already have the universal primers, you could try them on some representative specimens and see what results you'll get. That'll just cost you one PCR run + one gel or two. Regardless what primers you have, you should probably do that for all of them. You could of course develop your own primers, but that work may have already been done.
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