I am facing some problem in Glucose uptake assay using 2-NBDG. Can anyone provide me the exact protocol for the same?
Which method is best suited for the same assay, 2-NBDG or 18FDG? Please provide me the detailed protocol.
100-200 µg/mL 2-NBDG in glucose-free medium for 10-30 minutes after the addition of appropriate inhibitors (or sham controls), wash well 1-2 times, image immediately.
The protocol should done on Pre-adipocytes or differentiated adipocytes?
Either should work, so long as the cells are actively taking up glucose. This is a general protocol.
Once again thank you Mr. Jordan.
I think 2-3 days differentiated adipocytes will be better for the glucose uptake as well as other IHC & IF protocol.
Correct me if I am wrong.
hi, I performed glucose uptake assay using 2-deoxy-D-Glucose on 9 day old completely differentiated adipocytes and obtained good results. But this method is a conventional method.
Thanks for you answer...
Can you please provide me your detailed protocol?
my mail id is: [email protected]
I am facing problem regarding insulin dependent uptake, can anyone tell me the exact protocol for the same?
Hello all. Could anyone on this thread help me out with my questions related to 2-NBDG to measure glucose uptake in the link below: https://www.researchgate.net/post/About_protocols_used_for_glucose_uptake_in_HepG2_cells Any response is appreciate it. Many thanks
Bhavesh Variya did you manage to measure insulin stimulated glucose uptake? I am having similar problems. I'm trying to measure [14C]-2-deoxy-D-glucose uptake in differentiated 3T3-L1 cells but don't see any response to insulin. How old (days post differentiation or post differentiation initiation) are the cells when you do your experiments. And do you serum/glucose starve your cells? and also do you use DMEM with high or low glucose concentration?
I hope you can help out!
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