Bhavesh Variya

5 Questions 10 Answers 0 Followers

Questions related from Bhavesh Variya

Is it recommonded to replate differentiated 3T3-L1 adipocytes to 96 wll plate?

8-10 days mature adipocyte, seeded on 6 well plate could be transferred to 96 well or any suitable surface? How could I make adipocytes insulin resistant? Is it necessary to treat cell with...

14 February 2016 6,958 1 View

What is exact protocol for glucose uptake assay in 3T3-L1 cells?

I am facing some problem in Glucose uptake assay using 2-NBDG. Can anyone provide me the exact protocol for the same? Which method is best suited for the same assay, 2-NBDG or 18FDG? Please...

23 August 2015 9,855 10 View

Does anyone have colorimetric procedure to assess malic enzyme and fatty acid synthase activity?

I need some details to perform malic enzyme assay and fatty acid synthase using spectrophotometer or any other suitable methods.

19 March 2015 3,324 6 View

Does anyone have radiobinding protocol for PPAR-Gamma as well as Aalpha?

I would like to estimate binding of my new NCE with PPARs by any suitable procedures including radiobinding or transactivation assay. So, kindly provide the details for the above.

25 February 2015 1,026 1 View

How can I evaluate PPAR-Alpha activity? What is the procedure for estimation of various enzymes like CPT, FS, FAS, ME, G6PD and ACAT.?

I am focusing on PPAR-Alpha expression by estimating various enzyme activity (CPT, FS, FAS, ME, G6PD, ACAT etc.) from Rat Liver homogenates.. I don't have the exact procedure for estimation of...

10 December 2014 796 3 View