I am interested in the isolation of chicken primordial germ cells from embryo blood and I am following Yamamoto et al (2007) Article A Novel Method to Isolate Primordial Germ Cells and Its Use ...
. Here blood cells are suspended in the sodium citrate after extracting them from the embryo aorta. Can someone please recommend the alternate to sodium citrate? Can it be EDTA or DPBS?Thank you very much!
Hello
Sodium citrate would be ideal because it is not only an anti-coagulant but also serves as a buffer and a source of energy.
If not sodium citrate I would suggest you go for EDTA because EDTA is also an anti-coagulant, besides helping to preserve the morphology of cellular elements of blood.
DPBS is a balanced salt solution and not an anti-coagulant. So avoid using DPBS.
Best.
Dear colleaque the question clear,need the alternative of sod.citrate to suspend chicken blood,so the answer the following two alternatives are :
1 _Acid citrare dextroseACD Solution ,allow 1 part of ACD Solution to 3 parts of blood.
2_Alsevars solution,allow 1 part of Alsevar,s solution to 1 part of blood.
Best regards
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