Muhammad Abrar Yousaf

20 Questions 40 Answers 0 Followers

Questions related from Muhammad Abrar Yousaf

Reliable tool to identify transcription factors for genes?

I have a list of genes and would like to find transcription factors that regulate them based on experimental evidence. I have used some online tools, but they provide different results. Can anyone...

14 March 2025 6,072 3 View

What is an appropriate internal control during capillary electrophoresis for normalization in gene-specific cDNA synthesis?

I am analyzing the differential expression of splicing variants, through capillary electrophoresis, of a DNA repair gene after treatment with a DNA-damaging drug. I am using gene-specific primers...

09 December 2024 2,915 4 View

Is a template with low query coverage but high similarity suitable for protein modeling?

I am interested in predicting the protein structure of my protein of interest. Using NCBI BLAST, I found an experimental structure that corresponds to a domain of my protein, showing 24% query...

14 June 2023 8,849 3 View

How can I extract lowest binding energy from hundreds of AutoDock Vina docking log files?

I have performed multiple ligand molecular docking using AutoDock Vina in windows. There is any way to extract lowest binding energy value from all log files rather than copying value by opening...

14 January 2023 2,187 1 View

Is there any computational tool/webserver to find interactions between chemical compounds (other than proteins)?

I am interested to find interactions between plant wax and other chemical compounds. Is there any computational tool or webserver to find the interactions? Thank you

21 October 2022 9,708 4 View

Can someone please provide me any manual/tutorial to analyze the RAPD binary data using POPGENE software?

I am working on genetic diversity by RAPD. Can someone please provide me any manual/tutorial to analyze the RAPD binary data using POPGENE software? Thank you very much!

01 September 2021 9,417 4 View

What is alternative to sodium citrate for suspending the chicken blood cells?

I am interested in the isolation of chicken primordial germ cells from embryo blood and I am following Yamamoto et al (2007) A Novel Method to Isolate Primordial Germ Cells and Its Use ... . Here...

03 August 2021 9,381 3 View

Which is the best genetic marker for the assessment of inter- and intra-specific variations in fish?

I am interested in assessing the inter- and intra-specific variations in fish using genetic markers. RAPD, cytochrome b, COI, microsatellites are mostly used for the evaluation of genetic...

26 March 2021 8,750 7 View

Can mitochondrial gene COI be used for the identification of hybrid fish?

DNA barcoding is mostly being performed using the mitochondrial COI gene. As this gene is maternal, can it be used for the DNA identification of interspecific hybrid fish? Thank you in anticipation.

25 March 2021 3,409 3 View

Can principal component analysis (PCA) be explained by just one component as only one eigenvalue is > 1?

I am applying principal component analysis (PCA) on my data of morphometry but as the result of analysis I got just one eigenvalue greater than 1. So, is it possible to consider just one component...

18 June 2020 2,596 22 View

How to apply size effect removal from measurements of morphometrics by PAST software?

I am using PAST software to apply on morphometrics of fish. Can anyone tell me can I remove size effect using PAST as there is option present "remove size from distances" with ''allometric vs...

19 February 2020 7,534 5 View

Between application of formula or superimposition which one is better to use to remove the size effect in geometric morphometrics?

During determining the geometric morphometrics using TRUSS network, the size effect has been removed either by applying different formulas or by superimposition in the literature. Which one of the...

06 February 2020 5,633 2 View

How can I extract lengths between landmarks generated from tpsDig2 for fish morphometrics in TRUSS network?

I am interested in determining fish morphometrics by TRUSS network. Now how can I extract length/measurements by PAST software amonglandmarks on fish generated by tpsDig2 software? Can you please...

20 January 2020 2,669 15 View

Do anyone has any document/ file/ link for complete manual for morphometric characters using landmarks in TRUSS network?

I am interested to determine the morphometric characters of fish using TRUSS network system. Can anyone please suggest me the software and theirs manual to make landmarks and determine the...

17 January 2020 8,362 4 View

Can an animal sample e.g., fish specimen be preserved in commercial alcohol for DNA isolation in future?

For preservation of fish sample to isolate DNA in future, absolute or other concentrations of alcohol are used. Can we preserve the specimen in commercial alcohol which is available in...

15 January 2020 8,798 4 View

What should be used as control/blank during Cr (VI) detection by 1,5-diphenylcarbazide (EPA 7196A)method?

I am looking for the detection of Cr (VI) during experiment of Cr reduction by bacteria using 1,5-diphenylcarbazide (EPA 7196A) method. So, can anyone suggest me that the control/blank in the...

18 October 2019 2,677 4 View

Molecular Dynamics (MD) Simulation at 200 ns for co-authorship

My team and I are working on virtual screening against a viral protein. We are looking for a collaborator who can perform MD simulation of native protein and top three protein-ligand complex at...

01 January 1970 6,168 1 View

How to find homologous protein sequences and identify their conserved regions?

I'm interested in studying specific missense mutations in a human gene. My goal is to determine whether the mutated region of the protein is conserved across various species. Could you please...

01 January 1970 4,205 7 View

How can we relate the effect of water pollution with mitochondrial genes like cytochrome b in fishes?

Mitochondrial gene i.e., cytochrome b (cyb) in fishes is used for identification as well as for interspecific and intraspecific relationships and diversity. How can I relate the effect of...

01 January 1970 509 4 View

Do the aquatic pollution affect fish morphometrics?

I am looking for literature about the effect of aquatic pollution on fish morphometrics. Kindly may you suggest me the relevant literature. Thank you.

01 January 1970 1,004 1 View