I want to remove remove urea from peptide solution (elution solution from gel filtration column).The solution is 6M urea and 20mM tris (pH 7). I am trying to avoid dialysis as there are many peptides below 3kd.
You can try some chromatography methods, that would depend on the composition of your solution, the size of your peptides, their charge etc. Do you have any idea of the weight of your smallest peptide?
Otherwise, there are some non-specific adsobents that can remove urea ( no idea on their effect on peptides), urolytic enzymes etc, but i guess that the best would be a chromatography.
I basically agree to the chromatography idea, if the separation specificity is good enough (maybe based on hydrophobicity). You may also try to use an ultrafiltration membrane with a cut-off of 1 kDa (instead of 3) for a stirred cell or centrifugal ultrafiltration, which might be rather easy to establish, if the peptide sizes are at least around 2 kDa. Some electrochemical or enzymatic methods are probably not optimal for diluted peptide solutions.
I think you could use reverse phase cromatography to adsorb the peptides while urea passes by.
Thank all for their recommendations
Dear Pata the least size of peptide is 609 Da.
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