Isolate total DNA, quantify (a260/280/230 or Picogreen), then PCR (traditional+gel+densitometry or real-time) for a short (

Thanks a lot Gin,

I was indeed thinking to isolate mitochondria prior to DNA extraction, but if the yield is not ok I will go for a total extraction.

Have a nice day,

Michele

Hi Michele;

Did you find good protocol to measure mitochondrial DNA content ? (extraction, Primer sequences ...etc.

Thanks

Hello Abbas,

 my lab published this paper: Deletion of the Mitochondrial Chaperone TRAP-1 Uncovers Global Reprogramming of Metabolic Networks,

in the supplementary material there is the medthod used for mtDNA measurement. For simplicity, I attached it here.

Let me know if you need anything else, good luck,

Michele

Thank you Michele.

Abbas