Michele Tavecchio

3 Questions 6 Answers 0 Followers

Questions related from Michele Tavecchio

Any advice on the isolation of CD45 positive cells from B16F10 melanoma tumor?

Hello to everybody, I want to isolate all the leukocyte populations (CD45) from B16F10 tumors (injected in mice subcutaneously). Can someone recommend a good dissociation media (like PBS or...

20 November 2015 1,194 2 View

What is a good protocol for mitochondrial DNA measurement?

I would like to measure the mitochondrial DNA content to see differences after its depletion. Does anybody have a good protocol and some advice to share? Thank you.

20 November 2013 7,142 5 View

Can someone recommend a protocol for a myeloid-derived suppressor cells?

I am trying to differentiate bone marrow cells into MDSCs. After BM flushing, I plate them in complete RPMI at 2.5X10^5/mL with IL-6 and GM-CSF. Unfortunately, 24 hours later, the majority of them...

22 October 2013 8,657 19 View