What happens to the miRNA-RISC complex after it cleaves on a mRNA molecule?

Sammy Mustafa @Sammy_Mustafa

10 October 2019 1 9K Report

I'm more focused on the miRNA than RISC. Does the miRNA degrade after cleaving mRNA?

Andrey S Bavykin

yes, if not locked

Badges
Science topic

More Sammy Mustafa's questions See All

When the miRNA is bound to a mRNA, is it expressed in a miRNA PCR?

I conducted a miRNA PCR and I was wondering if miRNA that is bound to a mRNA is expressed in the results from my PCR. Thank you!

08 September 2019 7,517 7 View

Can pseudogenes affect miRNA gene expression or do they just competitively bind to miRNA?

The pseuodgene I'm researching has affected the miRNA levels in my cell culture (I found out through qPCR). However, I thought that pseudogenes just bind to the miRNA rather than affect its gene...

07 August 2019 2,012 2 View

What is the recommended concentration range of miRNA cDNA to use in a qPCR?

I am currently using the "qSTAR miRNA qPCR Detection System" protocol. I have isolated my miRNA cDNA, with my control concentration being 0.852 μg/μl and my transfected concentration being 0.637...

06 July 2019 4,365 5 View

How do you locate the exact spot of the scratch on a scratch assay?

I was wondering if anyone had any tricks to locate the exact spot of the scratch for a scratch assay. For example, I know some people in my lab have drawn a grid on their plates. Thank you!

06 July 2019 1,474 2 View

Will the PTENP1 plasmid transform properly in NEB 5-alpha Competent E. coli?

Addgene recommends the growth strain to be DH5alpha but we have NEB 5-alpha in my lab. Please let me know if my bacterial transformation can still work! https://www.addgene.org/35008/

11 December 2018 4,052 1 View

What transfection reagent should I use for the T98G cell line?

I am trying to do a stable transfection in the T98G cell line. I want a transaction reagent with a high transfection efficiency. A list of reagents would be preferable. Thank you!

08 September 2018 1,204 0 View

How do I get transfection efficiency with a plasmid with an HA tag?

I want to use a plasmid that is HA tagged. My research is in vitro and I am bit confused on what to do. Thank you!

08 September 2018 6,859 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View