What happens to the miRNA-RISC complex after it cleaves on a mRNA molecule?

I'm more focused on the miRNA than RISC. Does the miRNA degrade after cleaving mRNA?

09 October 2019 9,280 1 View

When the miRNA is bound to a mRNA, is it expressed in a miRNA PCR?

I conducted a miRNA PCR and I was wondering if miRNA that is bound to a mRNA is expressed in the results from my PCR. Thank you!

08 September 2019 7,420 7 View

Can pseudogenes affect miRNA gene expression or do they just competitively bind to miRNA?

The pseuodgene I'm researching has affected the miRNA levels in my cell culture (I found out through qPCR). However, I thought that pseudogenes just bind to the miRNA rather than affect its gene...

07 August 2019 1,959 2 View

What is the recommended concentration range of miRNA cDNA to use in a qPCR?

06 July 2019 4,290 5 View

Will the PTENP1 plasmid transform properly in NEB 5-alpha Competent E. coli?

Addgene recommends the growth strain to be DH5alpha but we have NEB 5-alpha in my lab. Please let me know if my bacterial transformation can still work! https://www.addgene.org/35008/

11 December 2018 3,982 1 View

What transfection reagent should I use for the T98G cell line?

I am trying to do a stable transfection in the T98G cell line. I want a transaction reagent with a high transfection efficiency. A list of reagents would be preferable. Thank you!

08 September 2018 1,123 0 View

How do I get transfection efficiency with a plasmid with an HA tag?

I want to use a plasmid that is HA tagged. My research is in vitro and I am bit confused on what to do. Thank you!

08 September 2018 6,804 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View