I am trying to do a stable transfection in the T98G cell line. I want a transaction reagent with a high transfection efficiency. A list of reagents would be preferable. Thank you!
I'm more focused on the miRNA than RISC. Does the miRNA degrade after cleaving mRNA?
09 October 2019 9,355 1 View
I conducted a miRNA PCR and I was wondering if miRNA that is bound to a mRNA is expressed in the results from my PCR. Thank you!
08 September 2019 7,517 7 View
The pseuodgene I'm researching has affected the miRNA levels in my cell culture (I found out through qPCR). However, I thought that pseudogenes just bind to the miRNA rather than affect its gene...
07 August 2019 2,012 2 View
I am currently using the "qSTAR miRNA qPCR Detection System" protocol. I have isolated my miRNA cDNA, with my control concentration being 0.852 μg/μl and my transfected concentration being 0.637...
06 July 2019 4,365 5 View
I was wondering if anyone had any tricks to locate the exact spot of the scratch for a scratch assay. For example, I know some people in my lab have drawn a grid on their plates. Thank you!
06 July 2019 1,474 2 View
Addgene recommends the growth strain to be DH5alpha but we have NEB 5-alpha in my lab. Please let me know if my bacterial transformation can still work! https://www.addgene.org/35008/
11 December 2018 4,052 1 View
I want to use a plasmid that is HA tagged. My research is in vitro and I am bit confused on what to do. Thank you!
08 September 2018 6,859 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View