Additives in organic solar cells?

Why additives are used in organic solar cells? Do they really participate in enhancing the performance? Don't they go off during drying or annealing? How their existence can be ensured after these...

26 March 2019 6,515 4 View

What is the excitation rate of an organic semi-conductor molecule under solar irradiation?

Hello researchers, What could be the excitation rate (in Hz) of a common organic dye/organic semiconductor molecule under sunlight? Could it be around 1000Hz or not? Also, if the molecule has 2...

23 July 2018 8,503 3 View

Weighing the variables in Response Surface Methodology with mixture models in process optimization, How?

In using mixture models with response surface methodology in determining the optimum conditions for a process, statisticians use weighed variables with the trend for maximizing, minimizing or to...

26 September 2017 2,658 2 View

Any suggestions on Western blotting troubleshooting on MW markers?

In Western blotting, sometimes the band obtained for an antibody is not in the region of its MW (specified by the manufacturer) according to the marker. But if we get a single band and used in a...

18 February 2015 2,464 6 View

What are the best methods to determine oxidative stress status of rodent tissues?

Need to know the best method to study the oxidative status of the mouse heart and kidney during pathological conditions.

29 January 2015 1,411 8 View

Will the RIN value decrease if RNA is isolated from FFPE Blocks??

As I need to isolate RNA from FFPE blocks using kit, i need to know the valuable answers before I start with the procedure. Pls share your experience if any

01 January 1970 335 3 View

Could you suggest the use of type of antibiotics during gestation period that would influence the immunity of fetus ?

01 January 1970 1,351 3 View

Contnuous or change-over trial, which one is better? Lets discuss

Please share your experience regarding continuous and change-over animal feeding trials. Through literature survey, I find that both have equal importance and shows to have certain advantages and...

01 January 1970 5,355 3 View

If any one having JCPDS software?

01 January 1970 2,933 55 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View