Additives in organic solar cells?

Why additives are used in organic solar cells? Do they really participate in enhancing the performance? Don't they go off during drying or annealing? How their existence can be ensured after these...

26 March 2019 6,515 4 View

What is the excitation rate of an organic semi-conductor molecule under solar irradiation?

Hello researchers, What could be the excitation rate (in Hz) of a common organic dye/organic semiconductor molecule under sunlight? Could it be around 1000Hz or not? Also, if the molecule has 2...

23 July 2018 8,503 3 View

Weighing the variables in Response Surface Methodology with mixture models in process optimization, How?

In using mixture models with response surface methodology in determining the optimum conditions for a process, statisticians use weighed variables with the trend for maximizing, minimizing or to...

26 September 2017 2,658 2 View

Any suggestions on Western blotting troubleshooting on MW markers?

In Western blotting, sometimes the band obtained for an antibody is not in the region of its MW (specified by the manufacturer) according to the marker. But if we get a single band and used in a...

18 February 2015 2,464 6 View

What are the best methods to determine oxidative stress status of rodent tissues?

Need to know the best method to study the oxidative status of the mouse heart and kidney during pathological conditions.

29 January 2015 1,411 8 View

Will the RIN value decrease if RNA is isolated from FFPE Blocks??

As I need to isolate RNA from FFPE blocks using kit, i need to know the valuable answers before I start with the procedure. Pls share your experience if any

01 January 1970 335 3 View

Could you suggest the use of type of antibiotics during gestation period that would influence the immunity of fetus ?

01 January 1970 1,351 3 View

Contnuous or change-over trial, which one is better? Lets discuss

Please share your experience regarding continuous and change-over animal feeding trials. Through literature survey, I find that both have equal importance and shows to have certain advantages and...

01 January 1970 5,355 3 View

If any one having JCPDS software?

01 January 1970 2,933 55 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View