How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

How to preserve wet fungus spore ?

I need to preserve broth culture of wet fungus spore for my further research. Can any one suggest me any solution.

18 January 2021 4,756 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Matlab: train random forest with time series? How to transform time series data to a supervised learning dataset?

Hey, I've build random forest using Matlab Machine-Learning Toolbox Function (treeBagger). I've computed several kinematic features like velocity or acceleration as predictors (24 predictors) for...

22 December 2020 2,638 3 View

Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes?

Why some basidiomycete cultures died quickly when stored at 5C on plate or slopes? and is there any method to return them back to life ? specially if the culture produce anamorphic stage ? and...

09 December 2020 4,631 3 View

Buffy Coat preparation? What freezing media is best for ensuring optimal integrity?

Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...

17 November 2020 1,899 3 View

Which isotopes can be used for preserving agricultural products?

Different kinds of Isotopes are playing very important roles in agriculture. They are used to detect different nutrient elements in soil and other crop improvement program. But which isotopes and...

16 November 2020 9,248 7 View

Cell culture techniques: how to deactivate a viral infection while preserving cytokine storm markers?

Hello, I am trying to measure the cytokine storm markers following SARS-Cov-2 viral infection of lung cells and macrophages under different conditions. However, finding a safe way to deactivate...

05 November 2020 1,174 4 View

Tiff vs JPEG?

The literature asserts that images taken in the RAW form and edited in a form like TIFF preserve image properties that are lost when edited in JPEG form. I have been experimenting with this and...

01 November 2020 5,727 3 View