Additives in organic solar cells?

Why additives are used in organic solar cells? Do they really participate in enhancing the performance? Don't they go off during drying or annealing? How their existence can be ensured after these...

26 March 2019 6,515 4 View

What is the excitation rate of an organic semi-conductor molecule under solar irradiation?

Hello researchers, What could be the excitation rate (in Hz) of a common organic dye/organic semiconductor molecule under sunlight? Could it be around 1000Hz or not? Also, if the molecule has 2...

23 July 2018 8,503 3 View

Weighing the variables in Response Surface Methodology with mixture models in process optimization, How?

In using mixture models with response surface methodology in determining the optimum conditions for a process, statisticians use weighed variables with the trend for maximizing, minimizing or to...

26 September 2017 2,658 2 View

Any suggestions on Western blotting troubleshooting on MW markers?

In Western blotting, sometimes the band obtained for an antibody is not in the region of its MW (specified by the manufacturer) according to the marker. But if we get a single band and used in a...

18 February 2015 2,464 6 View

What are the best methods to determine oxidative stress status of rodent tissues?

Need to know the best method to study the oxidative status of the mouse heart and kidney during pathological conditions.

29 January 2015 1,411 8 View

Will the RIN value decrease if RNA is isolated from FFPE Blocks??

As I need to isolate RNA from FFPE blocks using kit, i need to know the valuable answers before I start with the procedure. Pls share your experience if any

01 January 1970 335 3 View

Could you suggest the use of type of antibiotics during gestation period that would influence the immunity of fetus ?

01 January 1970 1,351 3 View

Contnuous or change-over trial, which one is better? Lets discuss

Please share your experience regarding continuous and change-over animal feeding trials. Through literature survey, I find that both have equal importance and shows to have certain advantages and...

01 January 1970 5,355 3 View

If any one having JCPDS software?

01 January 1970 2,933 55 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View