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For my IFA, I am using Alexa Fluor conjugated secondary antibodies. In my lab, other antibody, which is Cy3 conjugated has been kept at - 20 °C in aliquots. However, on the company data sheet, it...
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In my experiment, I use a plasmid (~16kb). What I want to do is to delete Luciferase gene from that plasmid by using a specific pair of primers with PCR technique. Last time, I tried and I got no...
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I have read an article in which the author mentioned that the cell lysates were mixed with the primary antibody before loading into the gel, then transferred to the membrane and probed with the...
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When AAV is produced in HEK cells, the helper gene, plasmid carrying packing system, and trans gene are used. AAV carrying trans gene is produced in a package. But, we also use AAV as a plasmid in...
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Hello everybody, I would like to ask you any protocols for Dengue Virus Propagation. Thanks.
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