11 May 2016 1 2K Report

Is there an advantage in keeping HT in the optimem-Glutamax culture media?

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What is going wrong with PCR? Works perfectly for One-Step RT but fails if I try to divide into separate reactions?

Hi all, Hoping for some troubleshooting/advice. Currently setting up a virus typing assay for routine use. I have the assay working with good sensitivity (regularly amplifies samples that are...

09 June 2024 4,468 3 View

What is an injectable buffer for mice around pH10?

Hello, In the lab we use baytril (enrosite) as an antibacterial post surgery. It comes ready to inject for horses but we need to dilute it for our mice to give them the correct dose. The thing...

30 April 2024 2,845 0 View

Is Gravity Chromatography an alternative for Size Exclusion Chrom for Mass Spectrometry?

currently conducting a study trying to figure out the secondary metabolites from bacteria. Question #1: does it make sense if our flow goes from: extraction > TLC > SEC > TLC >...

23 April 2024 3,603 1 View

Does Enzyme Concentration Affect Km?

Hello, I am using Trypsin to determine its Km and Vmax values against casein from bovine milk. I know higher enzyme concentrations increase the Vmax value, but does more trypsin also affect the...

04 April 2024 8,608 3 View

Michaelis-Menten Equation and Substrate Concentration?

Hello, Background: I am trying to find the Vmax and Km values for 0.25% trypsin and the substrate casein. During optimization, I have tried different time points (1-15 minutes) and different...

01 April 2024 1,553 1 View

What do I do if my Bradford Reagent yields underestimated protein concentration for measuring trypsin?

I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...

30 March 2024 8,491 0 View

Completely wrong plasmid sequence in transformants?

I have transformed NEB 5-alpha cells with a plasmid that I have pre-sequenced and confirmed it has the correct sequence. All I want to do is make more of this plasmid. However, when I transform...

04 December 2023 8,171 3 View

How do i transport of primary mouse hepatocytes ?

Hello, i have to transport some primary mouse hepatocytes in some 6 wells plates at 37 degrees celsius ideally. It's going to be a 45 min drive approximately. Does anyone have any ideas or...

06 November 2023 4,039 1 View

Does teaching infants sign language help the language acquisition process?

Research Design Project

10 September 2023 9,297 0 View

Any recommendations for Freshwater Fish of Costa Rica/Central America Literature Resources?

Howdy folks, I will be teaching a study abroad course in Costa Rica this summer (Caribbean side). My main research project with students will focus on freshwater turtles, but we anticipate...

06 February 2023 8,325 4 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

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Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

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