What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Can anyone help me troubleshoot QPCR for AAV titering?

So the lab that I work in generates a lot of AAVs and we titer them via QPCR. However, I've been noticing that the entire time, the efficiency has always been very low (anywhere from 40-80%) with...

22 November 2020 4,580 3 View

Can plant tissues be stored at -20C for DNA extraction?

Hey everyone. I collected some leaf tissue samples from the plant Phragmites australis from which I am hoping to extra DNA for sequencing. I will be extracting DNA using Qiagen DNeasy Plant Mini...

08 November 2020 4,989 12 View

Does anyone know of a marker expressed on all lymph-resident cells that can be used in flow cytometry?

I am looking for a marker found on all cells that reside in the lymph node - I study the immune response in salivary glands and we want to gate out any lymph resident cells to ensure that there is...

13 October 2020 6,322 2 View

Can you expect to see changes in secondary structure in a MD simulation?

While running a simulation on any peptide or protein, is it possible for the secondary structures to change? For example, seeing a steady rise in helical content over the whole simulation?

30 September 2020 4,298 9 View

What are ideal personal desktop hardware specs for comparing models for time series analysis and machine learning?

I am researching trading strategies, particularly involving time series analysis, but also machine learning, on data sets that range as far back as 1950 and would like to compare the accuracy of...

15 July 2020 7,733 3 View

How do I determine if my Fst values are significantly different from 0 or between populations that are and are not separated by a highway?

I am trying to determine if a highway is too much of a barrier for shrews to cross and breed freely. What I have to work with is ~1080 bp of the mitochondrial cytochrome b gene (we did not do...

22 June 2020 7,647 1 View

What is the best wearable EEG recording device for research?

Hi there, I am searching a wearable EEG device with as many channels and the highest accuracy possible. I found some papers which reviewed/used the following devices, but still not sure which is...

19 May 2020 5,712 2 View

Help needed with the anatomy of a mouse knee. What tissue is this?

Hi all, I have been collecting cross-sections of a mouse knee joint, and I keep seeing some differently coloured, yellowish tissue on the anterior side of the joint, but I'm not too sure what this...

27 January 2020 5,792 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View