Tommye Jordan

3 Questions 5 Answers 0 Followers

Questions related from Tommye Jordan

What effect does HT add to cell cultures?

Is there an advantage in keeping HT in the optimem-Glutamax culture media?

11 May 2016 1,850 1 View

What is the effect/purpose of the sucrose and EDTA added to agarose in the original Ouchterlony Procedure?

The original published procedure for Ouchterlony gels included sucrose and EDTA in the agarose. Why? How does it effect the result?

19 April 2016 5,978 2 View

Has anyone had a high background using sucrose or seablock in the blocking step following monoclonal binding to the plate?

Using stand monoclonal attachment to plate, followed by 6x washes with PBST, the plate is blocked with 1.2% seablock followed with a 2%sucrose 4%OVO solution block. We have a high background with...

24 March 2016 5,836 3 View