What does it mean when transfected macrophages increase in size (forward scatter) and granularity (side scatter)?

05 May 2018 1 1K Report

Cells treated only the transfection agent or the transfection agent with the miRNA construct (meant for transfection), had a higher forward scatter and side scatter compared to cells that were treated with culture media.

João Miguel Nina

I agree with Charyguly Annageldiyev (at least, I don't have any other reasons to add)!

However, I think he made a slight typo... you gate singlets using FSC-W vs FSC-A or SSC-W vs SSC-A.

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