These 'secretion-inhibited' cancer cells would then be co-cultured together with myeloid cells so I am hoping the environment would not be toxic to both cell types.
Do you want to inhibit all the cytokines or just a certain of them? Your case depends on how many cytokines and which cytokines you will inhibit, because some cytokines are essential for the cell survival, especially in the cancer cells. You need to perform a pilot study.
The use of brefeldin A can inhibit the protein trafic!, include cytokines
Hello
Yes i agree with Han Zhang, some cytokines are essential for the cancer cell survival
I agree with Han Zhang, but I think that even if you can inhibit the secretion of cancer cells, it can still utilize the secretions of other cells when you do the coculture because there are many similarities of growth factors between normal cells and cancer cells, so I guess they will survive.
Hi This might help
Addition of certain steroids e.g. glucocorticoids may do the trick : I found this on line
Article Cytokine modulation by glucocorticoids: Mechanisms and actio...
Protein kinase D regulates cytokine secretion, you could knockdown expression or use inhibitors. These approaches don't kill my oesophageal Cancer cells. Different isoforms (pkd1/2/3) regulate different cytokines so you'll have to do a bit of research!
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