Dear Sharon Lee,
When looking at cells in man then use CD68 or CD14 or CD115 for identification of macrophages. CD206(mannose receptor) is considered a valid marker for IL-4 treated M2- polarized macrophages. For blood monocytes use the same markers for identifcation and then either CD16 or slan (antibody DD1) to identify the non-classical monocytes. You will have to test the fixative with the different markers to make sure that the respective epitopes are preserved. Two papers on monocyte subsets are attached for your information.
Best wishes
Loems
We use CD14 and CD16 for human blood monocytes, for in vivo human macrophages CLEC5A (pro-inflammatory) and CD163L1 (anti-inflammatory). I hope this information can help you.
We usually use CD14 and CD16 to differentiate between classical (CD14+ CD16-) and non-classical (CD14low CD16+) monocytes in human PBMCs. However, it's important to make sure that you are not including some contaminant neutrophils, especially if you are going to fix your cells for intracellular or intranuclear staining. To exclude neutrophils, we always include an anti-CD66b antibody. We also include a dump channel (CD19, CD3, CD56, and CD57). Hope it helps.
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