Ann-Kristin Diederich

5 Questions 6 Answers 0 Followers

Questions related from Ann-Kristin Diederich

Does anyone has an idea how I get always the same false insert for different ligations with gram positives?

Hi, I am experiencing some problems with my ligation. I amplify my gene of interests from Enterococcus (~1000bp) + restriction sites for BamHI and SacI + thrombinase sequence and His-tag. Cut it...

12 December 2014 9,779 5 View

Bacterial RNA of stationary phase produces very distinct CT?

I am quite new to real time PCR, so maybe I don't see my fault. I isolated RNA of bacterial cell culture of stationary phase and transcripted it to cDNA. The RNA looks fine on the gel. When I do...

10 October 2013 10,029 1 View

Program or tool for data base synchronization of uniprot entries to fasta sequences.

I have a table with 300 Uniprot entries from mass analysis and want to have the sequence data for each of the entries. Is there a tool with which that can be done automatically?

07 July 2013 1,040 2 View

Does Coomassie stain substances with just one or few rather primary amino groups?

Substance is stainable with Ninhydrin, but does not appear on SDS when stained with very sensitive coomassie staining.

02 February 2013 5,408 3 View

I want to do 2D preparative thin layer chromatography and stain with iod for detection. How likely is the probability that my lipids will oxidize?

For development I use a mixture of CHCl3, MeOH and H2O.

02 February 2013 2,086 2 View