Rasoul Shafiei

6 Questions 41 Answers 0 Followers

Questions related from Rasoul Shafiei

How can I calculate the specific growth rate and lag phase?

I am trying to calculate the specific growth rate for a species of Acetobacter. The substrate is glucose. Batch culture is used by regulating pH, temperature, and dissolved oxygen. According to...

03 March 2014 6,567 48 View

How can I determine that a cell population is under oxidative stress?

A bacterial strain has been grown under certain conditions, and catalase and superoxidase activity have been determined in exponential and stationary phase. During stationary phase, these enzyme...

09 September 2013 343 27 View

Is there any method for determination of ATP by Flow cytometry?

Generally, ATP content of bacterial cells is determined by using disruption of cells, and then ATP is quantified in the extract. This method can determine the ATP in a population. I would like to...

08 August 2013 2,126 1 View

What is the Extinction coefficient that is used in the determination of enzyme activity ?

Could you please clearly explain the extinction coefficient and its importance in enzymatic assays? Should we consider it for all type of enzyme assays?

07 July 2013 1,105 8 View

Can I use cell crude extract to determine the effect of an inhibitory compound on one of the enzymes?

I have used French press to disrupt bacterial cells. After ultracentrifugation, the supernatant was used for determination of enzyme activity (PQQ-Glucose dehydrogenase). Now, I would like to...

07 July 2013 1,428 12 View

What are the differences between cell aging and cell senescence?

Really, I would like to know the differences between these two terms from physiological point of view?

07 July 2013 8,964 25 View