I have done nanodrop on the samples, some were detected to be phenol contamination as seen in the yellow pellets but results were not consistent at all. I suppose yellow pellets should be protein instead of phenol as phenol oxidises easily.
Is nanodrop consistent and good at detecting protein or phenol contamination - however my yellow pellets were not detected as protein contamination?
Dear Raymond,
depending on the sample you were extracting gDNA from and the method used it could be different kinds of contaminants. I was working on RNA extraction from akinetes and also experienced some kind of yellow pellet. Furthermore, you should be aware that the nanodrop measures dissolved molecules - if there are pellets the contaminant is not dissolved.
I agree with Martin. Should see wich samples you are using for extration. The yellow color is most caused by oxidation. As said yogesh maybe is lipid contamination. To conclude if is protein should use specific metod for protein detection,as biuret method or nitrogen determination
Good Job
- Badges
- Science method