Any software that could detect recombination in bacterial whole genome data? How far could we interpret this data?

When do we normally look at recombination in a large dataset of bacterial genomes- is this particularly important for medical pathogen? How do we normally interpret this?

08 September 2016 4,551 3 View

Does evoglow plasmid work well with in vivo real-time imaging?

I am wondering if evoglow plasmid work well with animal whole body imaging as some say cyan-green fluorescent proteins do not work well in animals as they penetrate poorly. Any recommendations for...

03 April 2016 7,518 0 View

What could be the yellow pellets seen after phenol-chloroform genomic DNA extraction?

I have done nanodrop on the samples, some were detected to be phenol contamination as seen in the yellow pellets but results were not consistent at all. I suppose yellow pellets should be protein...

01 February 2016 1,827 3 View

How do I confirm/visualise the sporulation of bacteria?

I am interested in Clostridial spore formers and wondering what are the methods to confirm the bacterial sporulation besides using microscopy?

01 February 2016 8,463 3 View

Does anyone know how to identify antimicrobial peptides secreted by bacteria?

11 December 2015 4,403 6 View

Does anyone know how to identify unknown components of gases produced by bacteria?

Does anyone know how to identify unknown components of gases produced by bacteria?

11 December 2015 7,564 0 View

Has anyone worked on IEC6 with C. perfringens (cell culture experiement)?

As IEC6 will die in anaerobic environment, C. perfringens will form spores in aerobic environment, will heat-killing method suitable in this case?

09 October 2015 6,828 1 View

How do I heat kill Clostridium spp (including C. perfringens)?

Any recommended paper or protocols? Autoclaving seems like not a good way as it might detroy the cell walls and stuff-I need intact bacterial components.

09 October 2015 5,150 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View