09 September 2016 1 3K Report

Any software pipelines that are more user-friendly for biologists to assign GO terms for bacterial whole genome data?

More Raymond Kiu's questions See All
Any software that could detect recombination in bacterial whole genome data? How far could we interpret this data?

When do we normally look at recombination in a large dataset of bacterial genomes- is this particularly important for medical pathogen? How do we normally interpret this?

08 September 2016 4,716 3 View

Does evoglow plasmid work well with in vivo real-time imaging?

I am wondering if evoglow plasmid work well with animal whole body imaging as some say cyan-green fluorescent proteins do not work well in animals as they penetrate poorly. Any recommendations for...

03 April 2016 7,746 0 View

What could be the yellow pellets seen after phenol-chloroform genomic DNA extraction?

I have done nanodrop on the samples, some were detected to be phenol contamination as seen in the yellow pellets but results were not consistent at all. I suppose yellow pellets should be protein...

01 February 2016 1,957 3 View

How do I confirm/visualise the sporulation of bacteria?

I am interested in Clostridial spore formers and wondering what are the methods to confirm the bacterial sporulation besides using microscopy?

01 February 2016 8,742 3 View

Does anyone know how to identify antimicrobial peptides secreted by bacteria?

I have aliquoted soluble media of bacteria. Just want to know what is the best method to analyse the sample - might contain small/large antimicrobial peptides?

11 December 2015 4,532 6 View

Does anyone know how to identify unknown components of gases produced by bacteria?

Does anyone know how to identify unknown components of gases produced by bacteria?

11 December 2015 7,688 0 View

Has anyone worked on IEC6 with C. perfringens (cell culture experiement)?

As IEC6 will die in anaerobic environment, C. perfringens will form spores in aerobic environment, will heat-killing method suitable in this case?

09 October 2015 6,944 1 View

How do I heat kill Clostridium spp (including C. perfringens)?

Any recommended paper or protocols? Autoclaving seems like not a good way as it might detroy the cell walls and stuff-I need intact bacterial components.

09 October 2015 5,279 1 View

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